UV-Visible Spectrophotometric estimation of azithromycin and cefixime from tablet formulation by area under curve method

Author : Chaitanya A. Gulhane, Anuja S. Motule, Jagdish V. Manwar, Harigopal S. Sawarkar, Prashant V. Ajmire, Ravindra L. Bakal

Area under curve method was developed using UV-Vis spectrophotometer for simultaneous estimation of azithromycin and cefixime from tablet formulation by area under curve method. Two analytical wavelength ranges used were i.e. 219-224nm and 275-302 nm for simultaneous estimation of both the drugs from tablet formulation. The method was statistically validated for its linearity, accuracy, and precision as per ICH guidelines. The recovery study was performed at 80%, 100%, and 120% levels. Average recovery at these three levels was found to be 99.91%w/w and 100.11%w/w for azithromycin and cefixime, respectively. Observed linearity was found to be in the range of 2.5-15µg/ml for azithromycin and 2-12 µg/ml for cefixime. The precision of the method was ascertained by performing an assay of formulation in terms of intra-day (2 hr interval) and inter-day variation (3 different days). The result obtained in intra-day study was 99.94%w/v (azithromycin) and 99.96%w/v (cefixime), and in inter-day, the results were 99.84%w/v (azithromycin) and 99.80%w/v (cefixime). Both Intra and inter-day variations showed less %RSD values indicating the high grade of precision of this method. The recovery was found to be 99.91%w/w for azithromycin and 100.11%w/w for cefixime.

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