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Biological activity of Stemphylium Vesicarium: An endophytic fungus isolated from Portulaca Oleracea

Author : Jayashankar M, Tejaswini H.K and Mohammed Ali Saeed

Every plant species harbors endophytes that can produce novel bioactive compounds. In this present study, an attempt was made to isolate endophytic fungi from edible leaves of Portulacaoleracea (Pursley) to determine its antibacterial, antioxidant, and antidiabetic activity. The antibacterial activity was done against human foodborne pathogens (Enterococcus faecalis, Escherichia coli, Salmonella typhi, and Shigella sonnei), with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)by microdilution broth method which, showed a good zone of inhibition against Salmonella typhi by Stemphylium sp. The MIC value between 10-20µg/ml and MBC value 20-40µg/ml was determined. The antioxidant activity was evaluated by DPPH, TPC, and FRAPassay, which showed considerable variations with reference to their positive control. The correlation among antioxidant assay indicated strong correlation between TPC and DPPH (R = 0.987, P < 0.01).While the antidiabetic assay by alpha-amylase inhibition was found to be 14.39-54.45%. The present study suggests the potential of endophytic fungi of Portulaca oleraceaas as a source for antibacterial, antioxidant, and antidiabetic agents.


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