Validated UV-Spectrophotometric method for quantitative estimation of Lomefloxacin HCl in bulk and pharmaceutical dosages forms

Author : Swatantra Bahadur Singh, Shweta Singh

The objective of this research was to develop and validate a simple, accurate, and precise UV spectrophotometric method for the determination of Lomefloxacin HCl in bulk and pharmaceutical dosage form .The λmax of the drug was found to be 287.1 nm in water. The beerís law was obeyed in the concentration range of 2- 12μg/ml with correlation coefficient 0.9997. The developed method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of the relative standard deviation and % recovery were found to be 0.2133 and 98.54- 99.85% respectively. On the other hand estimation of the drug in marketed formulation (Lomflox 400 mg tablets) was carried out by using the above developed and validated method. The percentage assay of tables was found to be 99.61 % in good agreement with the labeled claim, indicating the absence of interference of the excipient. All the results were good, showed that the proposed method is precise, accurate, hence can be used for the routine analysis of Lomefloxacin HCl in bulk and pharmaceutical formulation in both research laboratories, and pharmaceutical and chemical industries.

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