Using of paramagnetic nanoparticales with immunomagnetic bead ELISA in diagnosis of hydatidosis (Echinococcus Granulosus)
Cystic echinococcosus (CE) is a serious public health problem in sheep-raising regions. Detection of E. granulosus antigens is a better immunodiagnostic tool than determination of the antibody level. The circulating protoscolex antigen (CPA) used was obtained from lung and liver cysts of sheep and camel and injected in rabbits to raise specific polyclonal antibodies (pAb) against E. granulosus. A novel immunomagnetic bead ELISA based on immunoglobulin G (IgG) for detection of CPA in sera of rabbit infected with E. granulosus was developed. Detection of CPA in serum of human patients by sandwich ELISA gave a sensitivity of 90.48% and a specificity of 91.3% compared to other parasitic infections group and 100% compared to healthy control group. On the other hand, detection of CPA in serum of human patients by sandwich ELISA with paramagnetic nanoparticles gave a sensitivity of 95.2% and a specificity of 95.5% compared to other parasitic infections group and 100% compared to healthy control group. In conclusion, sandwich ELISA with paramagnetic nanoparticles techniques appears to be sufficiently sensitive assays for the detection of human echinococcosis antigen and valuable to judge the efficacy of chemotherapy than sandwich ELISA.
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