Serotyping and virulence potential of clinical Pseudomonas aeruginosa

Author : Ibtesam Ghadban Auda and Rajwa Hasen Essa

Pseudomonas aeruginosa considered as an important pathogen that have the ability to produce virulence factors, like pyocin and biofilm. Objectives of the study are serotyping of clinical local P. aeruginosa, determination of the ability to produce biofilm and pyocin and detecting of pyocin genes and their expression in relation to pyocin production. Fifty P. aeruginosa isolates were collected from different hospitals in Baghdad during the period from October to December 2014. Serotypes of P. aeruginosa were detected by slide agglutination test. The production of pyocin was determined phenotypically and pyocin S genes were detected by Polymerase Chain Reaction (PCR). The ability of bacteria to produce biofilm was tested by two different methods. As conclusion, serotyping of P. aeruginosa isolates showed different serotypes. The O7 serotype is appear to be new serotype in Iraq. One third of local P. aeruginosa isolates are pyocin producers. Genotypic detection of pyocin is better than phenotypic perhaps due to failure of pyocin gene expression. local P. aeruginosa isolates can produce both virulence factors, pyocin and biofilm, increase by that their virulence.

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