Method development and validation for simultaneous estimation of rabeprazole and levosulpride in bulk and pharmaceutical dosage forms

Author : Challa Sudheer, B. Tirumaleswara Rao and C. Rambabu

A rapid, sensitive, efficient and reproducible method for the determination of rabeprazole and levosulpride has been developed using reverse phase high performance liquid chromatographic method. This method involves separation of rabeprazole and levosulpride on a reversed phase Aligent, Zorbax C18 column (150 mmx4.6 mm I.D; particle size 5m) using UV detection at 221nm. The elution was done using a mobile phase consisting of buffer and acetonitrile in the ratio of 600:400 v/v on Shimadzu LC-2010 HPLC equipment. An external standard calibration method was employed for quantitation. The developed RP-HPLC method of rabeprazole and levosulpride was validated with respect to linearity, accuracy, precision, specificity and robustness respectively.

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