Method Development and Determination of Levodopa in Pharmaceutical Dosage Forms by Visible Spectrophotometry
Two simple, accurate and economic spectrophotometric methods A and B have been developed for the determination of levodopa in bulk and dosage forms. These methods are based on the formation of chloroform soluble ion-associates in the presence of acidic dyes namely Bromo Phenol Blue, BPB (Method A) and Bromo Cresol Purple, BCP (Method B) exhibiting max at 418 nm each. These methods obey Beer’s law in the concentration range of 2.0-10.0 µg/mL for both the methods. The molar absorptivities are found to be 8.77x103 and 1.94x104 L/mol.cm for methods A and B respectively. These methods are found to be suitable for the assay of levodopa in pharmaceutical formulations.
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