In-vitro and in-silico analysis of spirulina extracts against rheumatoid arthritis disease: A therapeutic approach

Author : Krishna Kumar Das, Anjana Bhagat, Lopamudra Singha, Souvagyalaxmi Sahoo

The current investigation of a combinatorial approach of insilico and invitro which was carried out to identify and explore the role of existing drugs and phytochemical compounds against potential targets of RA. In this study we got 150 genes related with the RA disease. From that 18 genes (proteins) were mostly related to 9 drugs to cure the disease. From the literature survey 8 Phytochemicals like Flavonoids, Alkaloids, Steroids, Lutein, Saponins, Tannin, Lycopene, and Alpha-Carotene were mostly present in Spirulina. These 4 proteins IL2RA, CFLAR, CASP8, and PTPN22 were mainly affected by Alkaloids, Flavonoids, and the Alpha-Carotene. Then the antibacterial activity of S. platensis was evaluated in different concentration of solvent extract (Methanol & Ethyl acetate) against targeted bacterial species (S. aureus and E.Coli). Toxicity of the solvent extract was tested by Paper disc diffusion method. Methanol extract gave the highest zone of inhibition than Ethyl acetate extract for the both bacteria E.Coli and S. aureus. FRAP-assay had been used to determine the antioxidant activity as it was simple and quick and the result shown that the Ethyl acetate extraction was more efficient than the Methanol extraction.

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