HPTLC finger printing profile of flower extract of Punica granatum

Author : R. Nalini, R. Anuradha and S. Kamalaveni

The main objective of the study is to investigate the fingerprint profile of Punic granatum using high performance thin layer chromatography (HPTLC) technique. Camag HPTLC system equipped with TLC Linomat V applicator, Camag TLC scanner III and winCATS software were used. Hydroalcoholic extracts of the flower of Punic granatum was developed in suitable mobile phase using standard procedures and the plates were kept in Photo-documentation chamber (CAMAG REPROSTAR 3) and captured the images at UV light at 254 nm and 366 nm. The fluorescent band (under 254 nm and 366 nm) at Rf 0.56 in mobile phase Toluene: Ethyl acetate: Formic acid (5:4:1) was found and the marker compound Quercetin was quantified.

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