Detection of Genes Encoding Pyocin Production of Pseudomonas aeruginosa

Author : Rajwa H. Essa, Hussain D. O. AL-Tamimi and Khetam H. Rasool

More than 90% of Pseudomonas aeruginosa strains are pyocins producer and each strain may synthesis several types of pyocins. pyocins displayed growth inhibitory activity against some Gram-positive and Gram-negative bacteria .Fifty bacterial isolates of Pseudomonas aeruginosa have been collected from many hospitals in Baghdad (Iraq) include: Al-Kindy hospital, Ibn Al-baladi hospital, wound and burn hospital and Baghdad hospital in medical city during the period from Sep. to Dec. (2014). These isolates were collected from different sources, they were diagnosed depending on morphological and microscopic properties as well as by using biochemical tests, and VITEK2 compact system, pyocins production was detected by using cross streaking method. The results showed that (16) isolates out of (50) isolates have the ability to produce pyocins. Polymerase Chain Reaction (PCR) technique used to detect gene responsible for S type pyocins (S1S2 pyocins and S3 pyocin), results appear that there were (24) isolates from all (50) isolates (48%) contained the S1S2 gene, (21) isolates (42%) contained S3 gene. The total number of isolates which contain genes encoding of S-type pyocin production (both S1S2 and S3 genes) were (31) isolates (62%).

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