Cytotoxicity and apoptosis of glioblastoma multiforme cell line by arginine deiminase purified from a higher productive isolation Enterococcus faecium M1

Author : Nada Z. Mahdi, Shatha S. Al-Tahan and Nahi Y. Yaseen

Purified arginine deiminase from Enterococcus faecium M1 isolate is the strongest cancer treatment enzyme due to its activity and stability in different environmental conditions. The cytotoxicity of ADI to glioblastoma multiforme (ANG) cancer cell line and rat embryo fibroblast(REF) normal cell line for (24, 48 and 72h) were estimated, the inhibition rate(IR) increased with raising of ADI concentration and incubation period for ANG cell line but these results were opposite for REF cell line that IR decreased with increment of incubation period. Different concentrations (2-1000ng) of enzyme were used, the significant once were between (30-100ng) that they were safe for most cells of REF normal cell line but they inhibited the large numbers of glioblastoma cells, that the IC50 of ADI was 37ng/ml for this cancer cell line during 72h of incubation time and the IR reached to 75.4% after 48h incubation with 100ng/ml of enzyme. The ability of ADI to produce intrinsic mitochondrial apoptosis effect on ANG and REF cell lines was investigated, the results showed that the main reason of cell cytotoxicity was the induction of apoptosis process by ADI enzyme and they were compatible to the results of cytotoxicity test. We concluded that ANG cancer cell line could not produce arginine amino acid thus it was highly sensitive to arginine deprivation by the robust activity of arginine deiminase enzyme, but it was safe for REF normal cell line could produce arginine during the incubation time with enzyme.

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