Comparative genomics of bacterial pathogens to design and validate a diagnostic PCR assay for detection of UPEC (UTI89)

Author : Shadia S. Alhamd and Manal I. Khaleel

Urinary tract infections (UTIs) present clinical problems which can be caused infection by billions of organisms, including Escherichia coli as the main common cause. This invasion triggers a series of risk factors such as kidney failure or patient death in a worst-case scenario. Infection occurs when bacteria enter the bladder by escaping at micturition or as a result of contamination; these bacteria will directly colonize the bladder. UTI are most commonly found in women, which is basically related to the anatomical features of the female urethra. This paper concentrates more on the main methods of detection of UTIs. The entiregenome was determined and sequenced using bioinformatics analysis, which includes the programs Virulence Gene, Island Viewer and, most importantly, Blast N. Comparisons were made among all three strains of Escherichia coli to indicate the target gene, which was subsequently used as a PCR primer target.

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