Bioactive Natural Products from Polyalthia Longifolia and its Fungal Endophyte Alternaria sp.
Medicinal plants and fungi are rich sources of natural products. The plant Polyalthia longifolia was investigated for its phytochemical components and its own endophytes. Phytochemical investigation of the aerial parts of P. longifolia has led to the isolation and identification of β-sitosterol-3-O-β-D-glucoside (1), 14,15-bisnor-3,11-E-kolavadien-13-one (2), 3-β,5-β,16-α-trihydroxyhalima-13(14)-en-15,16-olide (3), isoquercetin (4), rutin (5), and trans-cinnamic acid (6). Furthermore, the endophyte Alternaria sp. (MT995125) was isolated from the leaves and the compounds pyrenolide-D (7) and modiolide-A (8) were identified in its solid culture. The isolated compounds were tested against human epithelioid cervical carcinoma (HeLa), human colorectal carcinoma (HCT-116) and human amnion (WISH) normal cell line. The compounds showed moderate to strong cytotoxic activity with minimal toxicity towards normal cell line WISH compared to the positive control doxorubicin. The significant cytotoxic activities of some compounds were augmented by in silico strong inhibitory activity to carbonic anhydrase (IX isoform) (CA IX) through performing docking experiment.
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