Bio-autography guided screening of antimicrobial compounds produce by Enicostemma littorale Blume
The present study is attempt to evaluate the antimicrobial activity and to screen out the antimicrobial component produced by Enicostemma littorale Blume. Ethyl acetate used for the extraction. Disk diffusion method was used for antimicrobial activity and bio-autography was carried out for the detection of antimicrobial component. Bio-autography is a technique that combines thin layer chromatography with bioassay in situ. Extract of root and stem show the zone of inhibition against Escherichia coli, Pseudomonas auroginos, Bacillus subtilis, Salmonella typhi, Staphylococcus aureus due to the presence of flavonoid and flavones component determine by bio-autography.
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