Urinary tract infections (UTIs) present clinical problems which can be caused infection by billions of organisms, including Escherichia coli as the main common cause. This invasion triggers a series of risk factors such as kidney failure or patient death in a worst-case scenario. Infection occurs when bacteria enter the bladder by escaping at micturition or as a result of contamination; these bacteria will directly colonize the bladder. UTI are most commonly found in women, which is basically related to the anatomical features of the female urethra. This paper concentrates more on the main methods of detection of UTIs. The entiregenome was determined and sequenced using bioinformatics analysis, which includes the programs Virulence Gene, Island Viewer and, most importantly, Blast N. Comparisons were made among all three strains of Escherichia coli to indicate the target gene, which was subsequently used as a PCR primer target.

Keywords : Urinary tract infections, Escherichia coli, PCR primer target

Author : Shadia S. Alhamd and Manal I. Khaleel

Title : Comparative genomics of bacterial pathogens to design and validate a diagnostic PCR assay for detection of UPEC (UTI89)

Volume/Issue : 2020;8(8)

Page No : 52-59

The current investigation of a combinatorial approach of insilico and invitro which was carried out to identify and explore the role of existing drugs and phytochemical compounds against potential targets of RA. In this study we got 150 genes related with the RA disease. From that 18 genes (proteins) were mostly related to 9 drugs to cure the disease. From the literature survey 8 Phytochemicals like Flavonoids, Alkaloids, Steroids, Lutein, Saponins, Tannin, Lycopene, and Alpha-Carotene were mostly present in Spirulina. These 4 proteins IL2RA, CFLAR, CASP8, and PTPN22 were mainly affected by Alkaloids, Flavonoids, and the Alpha-Carotene. Then the antibacterial activity of S. platensis was evaluated in different concentration of solvent extract (Methanol & Ethyl acetate) against targeted bacterial species (S. aureus and E.Coli). Toxicity of the solvent extract was tested by Paper disc diffusion method. Methanol extract gave the highest zone of inhibition than Ethyl acetate extract for the both bacteria E.Coli and S. aureus. FRAP-assay had been used to determine the antioxidant activity as it was simple and quick and the result shown that the Ethyl acetate extraction was more efficient than the Methanol extraction.

Keywords : Rheumatoid arthritis (RA); S. platensis; Methanol & Ethyl acetate; S. aureus and E.Coli; FRAP-assay; Spirulina

Author : Krishna Kumar Das, Anjana Bhagat, Lopamudra Singha, Souvagyalaxmi Sahoo

Title : In-vitro and in-silico analysis of spirulina extracts against rheumatoid arthritis disease: A therapeutic approach

Volume/Issue : 2020;8(8)

Page No : 60-73

The present research work attempts to evaluate the antimicrobial activity of Ethanol and Methanol extracts of two traditional medicinal plants viz. Cinnamomum zeylanicum, Syzygium aromaticum. Since ethanol is the best solvent for dissolving the antimicrobial compound present as active phytoconstituents in plants, which extracted with ethanol. Each extract was tested for its antimicrobial activity against Eight most prevalent pathogenic microorganism Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Bacillus subtilis, Escherichia coli, Enterobacter aerogenes, Micrococcus luteus, Aspergillus niger The preliminary antimicrobial activity testing was carried out by using agar well diffusion method and minimum inhibitory concentrations of the extract was then determined by using the micro broth dilution assay. All these plant extracts exhibited antimicrobial activity against tested microbes the ethanol & methanol extract of Cinnamomum zeylanicum showed the highest antimicrobial activity against Micrococcus luteus & Staphylococcus aureus with the zone of inhibition 16 mm.

Keywords : Cinnamomum zeylanicum, Syzygium aromaticum, Antimicrobial Activity, Bacillus subtilis, Escherichia coli, Eneterobacter aerogenes, Micrococcus luteus, Aspergillus niger

Author : Dhara Harsoda, Roshani Chaudhari, Nikita Chavada, Dhaval Prajapati

Title : Antimicrobial activity of selected medicinal plants cinnamon and clove

Volume/Issue : 2020;8(8)

Page No : 74-81

Glaucoma is a chronic progressive optic neuropathy that causes irreversible damage. Therefore, early diagnosis and appropriate treatment to control various risk factors associated with glaucoma are important. Risk factors associated with the development of glaucoma have been reported by many researchers and include both ocular and non-ocular (systemic) factors such as myopia, central corneal thickness, disc hemorrhage, and genetic factors. However, little is known about the association between vitamin D and open-angle glaucoma (OAG). As a consequence, many glaucoma patients are diagnosed, monitored, and treated at an age amply beyond the age corresponding to their median life expectancy at birth. To make a proper estimate of the life expectancy of glaucoma patients, life expectancy should be adjusted for the age already reached: residual life expectancy.

Keywords : Optic Neuropathy, Ocular, Myopia, OAG, Median Life, Life Expectancy

Author : Dr. Shivani Awasthi

Title : The Relativity between Vitamin D and Glaucoma - A Cross Sectional Study from Rewa (M.P)

Volume/Issue : 2020;8(8)

Page No : 82-89

Papain is proyeolytic enzyme that catalyses the breakdown of a protein. It shows several biological functions in plants and animals. It use in many industry like food, leather,detergents, meat processing and medicinal application like blood coagulation, fibrinolysis and digestion process there are various process use for the extration of papain and purification also. Now a day the various formulation of papain is available in market in topical and other forms. Spoons of pulvevised papaya seeds mixed with hot water used in diabetes. The papain shows protelytic activity is determine by using various method and the previous studies reported activity in latex of papaya plant. Main objective of this study was to evaluated protease activity in the papaya nectar.

Keywords : Papain, Protelytic Enzyme, Papaya Isolation, Purification of Protein

Author : Jadhav Shreegita, Pranjal Barbade, Akshay More and Swapnil Nirgude

Title : Papain - An essential proteolytic plant enzyme

Volume/Issue : 2020;8(8)

Page No : 90-97

‘Power of Gratitude and positive psychology’ is a case study of a young chap of 28 years old. He narrated his story in his own words. He is the only child of a well-established and functional family. Despite the education and various luxuries, he wasn’t satisfied. He was heading towards moderate to severe depression. The counselor thought GQ-6 test would be appropriate for such a person. Unsurprisingly, he scored poorly on it. The problem seemed to be a lack of gratitude for what he had and a lack of direction in life. The attempt at a solution for the former is the premise of this paper. A kind of meditation, derived from the idea of ‘12 virtues’ and the theory ofinduction or more precisely, nudging of feelings through ambient color was picked up from Matrimandir, Puducherry. Light green color was found appropriate for the task. The total practice went on for 4- 5/7-day weeks. The score had improved positively. Guided meditation on gratitude which focused on noticing the good things already at hand helped him, and might help more like him, in feeling more positive, hopeful, and willing to take responsibility for improving what can be improved.

Keywords : Gratitude, Positive Psychology, Virtue, Guided Meditation

Author : Dr. Nishi Arora and Dr. Praveen Choudhary

Title : A Case Study: Power of Gratitude and Positive Psychology

Volume/Issue : 2020;8(8)

Page No : 98-100

Bullous Lichen Planus (BLP) is a rare form of inflammatory and immune mediated muco-cutaneous lesion characterized by vesicles or bullae that occur in the sense of pre-existing lesions of Lichen Planus (LP). Biopsy is the essential diagnostic criteria to conclude the clinical features of BLP. The histologic features in conjunction with the negative immunofluoresence indicate that bullous lichen planus is a form of "hyper-reactive lichen planus". Here, we report a case of Bullous Lichen Planus presenting with multiple aggressive and hyper-pigmented raised lesion and blisters over both upper limbs and back for the last 3 months. There is no standard treatment for bullous lichen planus. Management aims to control symptoms and to decrease time from onset to resolution. It involves topical corticosteroids, but varies depending on the severity and location of the lesion. The symptoms resolved with the treatment of Tricyclic H1 antagonist (Desloratadine) and Corticosteroids (Clobetasol and Betamethasone).

Keywords : Bullous Lichen Planus, Lesion, Bullae, Tricyclic H1 antagonist, Corticosteroid.

Author : Maneesha T, Gopika K S, Tonya Clara Thomas, Arathi T.V, Rebia V, Soniya TV

Title : Case report on bullous lichen planus

Volume/Issue : 2020;8(8)

Page No : 101-103

Lehana is one such traditional formulation mentioned in Ayurvedic classics that have been used to build up immunity, promote health, enhance growth and development by providing sufficient nutrition, protecting from various infections along with improving intellect and speech in children. Different Acharyas described several Lehana formulations for use in Balyaawastha. The immune system is a network of cells, proteins, tissues and organs that defends the body against infection. It is relatively immature at birth hence newborn are at greater risk of illness due to an immature immune system. So during this period our attempt should be specified to prevention of diseases and to enhance physical, mental and social well-being of children. In Ayurvedic texts immunity has been explained in terms of Vyadhikshamatva which is a state of equilibrium of Kapha, Bala, and Oja. Different therapies like Rasayan, Lehana, and Samskaras etc. are focused to modulate the immunity or increase the Vyadhikshamatva in children. Healthy state can be achieved by fine tuning of immune-regulatory mechanisms with the help of Lehana. The main aim of this article is to describe literary view of Lehana and its health benefits in children.

Keywords : Vyadhikshamatva, Oja, Lehana, Rasayan

Author : Arya Deepika, Pandey Reena

Title : Ayurvedic perspective of lehana and its immunomodulating effect in children

Volume/Issue : 2020;8(8)

Page No : 104-107

The COVID-19 pandemic caused by SARS-CoV-2 remains a significant issue for global health, ecomomy and society. SARS-Cov-2 (sever acute respiratory syndrome Coronavirus 2) epidemic has spread to virtually every continents in the world. These viruses get spread through airbone droplets from human to human or human to animal. Coronavirus cause respiratory infections in human and diarrhoea, Upper Respiratory disease in animals, person to person transmission of virus is occurred by contact with disease individual and droplet inhalation of injected person. This virus enters in human cell through membrane. ACE-2 expo peptidase receptor. The advice from WHO and ECDC to avoid public places and close contact with infected persons and animals.

Keywords : Corona virus, WHO, Emergency, Pandemic, Covid 19

Author : Akshay B. More, Manshi S. Pandit, Archana R. Gavit, Shrigeeta R. Jadhav

Title : Corona Virus: A Review on Covid-19

Volume/Issue : 2020;8(8)

Page No : 108-114

In recent years there is a growing the interest in Nutraceutical which is important for health promotion and disease risk reduction. Nutraceutical are food or part of food that provides medical or health benefits including the prevention and treatment of a disease. The principal behind the growth of the Nutraceutical market worldwide are the growth of population and the health trends. Nutraceutical which used in food product can be categorized as dietary fiber, prebiotics, probiotics, polyunsaturated fatty acids, antioxidants, Nutraceutical enzyme and different types of herbal or synthetic food. Nutraceutical has important advantage over the medicine because they avoid side effect, have naturally dietary supplement or dietary fiber etc. Nutraceutical and functional foods are assuming a middle ground between food and drugs due to growing body of evidence that supports to maintaining health and contributing to treatment of disease. “Traditional food” contains vitamins such as Vitamin- A, Vitamin-B, Vitamin-C and minerals which is useful in the diet. It is useful for supporting health and wellness. Whereas “functional foods” which have positive effect on health beyond basic nutrition. Nutraceutical help to cure some of the major health problems such as obesity, cardiovascular diseases, cancer, osteoporosis, rheumatoid arthritis, diabetes, cholesterol etc. Herbal Nutraceutical which is used as a powerful instrument in maintaining health and provide the protection against acute and chronic diseases, thereby promoting optimal health, longevity, increase life expectancy and support the function of the body. The present review focused on herbal Nutraceutical which is important for human health.

Keywords : Nutraceutical, dietary supplement, Functional food

Author : Pranali S. Satkar, Suraiya N. Shaikh

Title : Current Concept and Prospect of Herbal Nutraceutical

Volume/Issue : 2020;8(8)

Page No : 115-118

A simple, Precised, Accurate method was developed for the estimation of Abiraterone by RP-HPLC technique. Chromatographic conditions used are stationary phase Discovery C18 150mm x 4.6 mm, 5 ,Mobile phase 0.01% KH2PO4:Acetonitrile in the ratio of 60:40 and flow rate was maintained at 1.1 ml/min, detection wave length was 235 nm, column temperature was set to 30oC and diluent was mobile phase Conditions were finalized as optimized method. System suitability parameters were studied by injecting the standard six times and results were well under the acceptance criteria. Linearity study was carried out between 25% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 0.6 for repeatability and 0.2for intermediate precision. LOD and LOQ are 0.953µg/ml and 2.888µg/ml respectively. By using above method assay of marketed formulation was carried out 99.90% was present. Degradation studies of Abiraterone were done, in all conditions purity threshold was more than purity angle and within the acceptable range.

Keywords : HPLC Abiraterone, Method development. ICH Guidelines

Author : Anusha Jeripothula, Pavani Digigam and K. Abbulu

Title : Stability Indicating Method Development and Validation for Determination of Abiraterone in Bulk and Pharmaceutical Dosage Forms by RP-HPLC

Volume/Issue : 2020;8(8)

Page No : 119-125